Further, this method overcomes the problems associated with any viral-induced changes in host cells (e.g., binding and aggregation of host proteins) that might affect their assay by techniques such as Western blotting and elisa. ACV-resistant isolates were plaque purified and screened by Southern hybridization for disruption of.4 kilobase pair EcoRI fragment that encompasses the TK coding sequences.
Godowski PJ, Knipe DM (1985) Identification of a herpes simplex virus function that represses late gene expression from parental viral genomes. Alzheimer's Reports 1: 173178. Santana S, Recuero M, Bullido MJ, Valdivieso F, Aldudo J (2011) Herpes simplex virus type I induces the how to write a memo for research paper accumulation of intracellular beta-amyloid in autophagic compartments and the inhibition of the non-amyloidogenic pathway in human neuroblastoma cells. The other strain 17-derived viruses were titrated on baby hamster kidney (BHK-21) cells. HSV1 elisa 96-well plates were seeded with.20104 Vero cells/well and the cells allowed to settle overnight. However, stimuli such as stress and immunosuppression can reactivate the virus, leading to a productive infection. Infection with recombinant ts K /lacZ showed no A accumulation at the restrictive temperature (C). J Pathol 167: 365368.
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